Japan alcohol – NSMS 10 http://nsms10.com/ Thu, 29 Sep 2022 02:37:53 +0000 en-US hourly 1 https://wordpress.org/?v=5.9.3 https://nsms10.com/wp-content/uploads/2021/06/icon-71-150x150.png Japan alcohol – NSMS 10 http://nsms10.com/ 32 32 Coronavirus Morning News Brief – September 28: North Korea launches mass vaccination campaign, Japanese Covid pill shows promise https://nsms10.com/coronavirus-morning-news-brief-september-28-north-korea-launches-mass-vaccination-campaign-japanese-covid-pill-shows-promise/ Wed, 28 Sep 2022 16:01:50 +0000 https://nsms10.com/coronavirus-morning-news-brief-september-28-north-korea-launches-mass-vaccination-campaign-japanese-covid-pill-shows-promise/ Adirondack chairs in the Andironacks in upstate New York Hello. This is the report by Jonathan Spira. Now here is the pandemic news from around the world on the 902nd day of the pandemic. President Biden’s premature declaration that the pandemic was over and the clumsy pivot from the White House fueled a discussion about […]]]>

Adirondack chairs in the Andironacks in upstate New York

Hello. This is the report by Jonathan Spira. Now here is the pandemic news from around the world on the 902nd day of the pandemic.

President Biden’s premature declaration that the pandemic was over and the clumsy pivot from the White House fueled a discussion about how we will know when the pandemic is over. Apparently the fat lady hasn’t sung yet.

I am a trained historian and graduate and, much like when looking for when a paradigm shift took place, the end of the pandemic can only be determined in the rear view mirror.

There will not be a single moment like the armistice of November 11, 1918 at 11 a.m. when the First World War ended. We will look for milestones, including interrupting transmission of the virus, eliminating hospitalizations and deaths, and a broad relaxation of self-isolation.

At this point we have barely reached the third point.

We have made great progress but the coronavirus has always known how to be one step ahead and it is only when science is one step ahead of the virus that we can truly say that the pandemic is over.

In the news we’re covering today, Disney Cruises has ended vaccination mandates for most cruises, North Korea has embarked on a mass vaccination campaign, and a Japanese drugmaker has a promising drug. with which to deal with Covid.

Here’s a look at what’s happened over the past 24 hours.

UNITED STATES

New York State has figured out what to do with the 168 shipments of expired hand sanitizer it still has on hand since the early days of the pandemic. Prisoners in public facilities produced hundreds of thousands of gallons of hand sanitizer and much of it remains in storage at the New York State Preparedness Training Center in Oriskany and expired. The state has contracted Eastman Kodak to dispose and recycle the expired hand sanitizer and Kodak will distill it to extract the isopropyl alcohol for reuse in manufacturing. The project is expected to last 44 weeks.

OVERALL

North Korea has embarked on a mass vaccination campaign against the coronavirus, announced South Korea’s National Intelligence Service, its main intelligence agency. The spy agency did not say specifically which border areas began vaccinations or the vaccine supplier, although the doses are likely to have come from China. North Korea was one of two countries in the world that did not launch mass vaccination campaigns. The other is Eritrea in East Africa, whose neighbors include Ethiopia and Sudan.

In Japan, the pharmaceutical company Shionogi reported that its oral treatment for Covid demonstrated a significant reduction in symptoms compared to a placebo in a phase III trial in Asia.

TRAVEL

Disney Cruise Line will no longer require proof of vaccination for passage on most of its cruises, making it the latest major cruise line in the United States to eliminate the requirement. For voyages on Disney Wish, Disney Dream, Disney Fantasy and Disney Wonder starting Oct. 14, full vaccinations are “strongly recommended,” but not required, the cruise line said on its website. Proof of a negative coronavirus test taken within two days of travel is required for unvaccinated passengers aged five and over.

TODAY’S STATISTICS

Now here are the daily stats for Wednesday, September 28.

As of Wednesday morning, the world had recorded 621.6 million cases of Covid-19, an increase of 0.5 million cases and more than 6.5 million deaths, according to Worldometer, a service that tracks such information. Additionally, 601.6 million people worldwide have recovered from the virus, an increase of 0.4 million.

Worldwide, the number of active cases of coronavirus as of Wednesday is 13,256,190, an increase of 89,000. Of this figure, 99.7%, or 13,216,539, are considered mild and 0.3 %, or 39,651, are listed as critical. The percentage of cases considered critical has not changed in the past 24 hours.

The United States reported 41,906 new coronavirus infections for the previous day on Wednesday, compared with 58,520 on Tuesday, 5,275 on Monday, 8,091 on Sunday, 44,458 on Saturday and 92,729 on Friday, according to data from the US Department of Health and Human Services. social. . The 7-day incidence rate is now 51,249. Figures for the weekend (reported the next day) are typically 30%-60% of those shown on weekdays due to fewer tests being performed .

The average daily number of new coronavirus cases in the United States over the past 14 days is 51,259, a drop of 21%, according to data from the Department of Health and Human Services, among other sources. The average number of daily deaths over the same period is 415, a decrease of 4% over the same period, while the average number of hospitalizations over the period was 28,974, a decrease of 15%.

In addition, since the start of the pandemic, the United States on Wednesday recorded just under 98 million cases, a figure higher than any other country, and a death toll of 1.08 million. India has the second highest number of officially registered cases in the world, 44.6 million, and a reported death toll of 528,584.

The latest data from Russia’s state statistical service Rosstat showed that, at the end of July, the number of Covid- or Covid-related deaths since the start of the pandemic there in April 2020 is now 823,623, giving the country the second highest pandemic in the world. related death toll, behind the United States. Rosstat reported that 3,284 people died from coronavirus or related causes in July, compared to 5,023 in June, 7,008 in May and 11,583 in April.

Meanwhile, France is the third country with the highest number of cases, with 35.2 million, although Brazil recorded the third highest number of deaths from the virus, 685,930, and recorded 34.7 million cases, placing it fourth. insert.

Germany ranks fifth with 33.1 million cases.

The other five countries with total cases above 20 million are South Korea, with just under 24.7 million cases, the United Kingdom, with 23.6 million cases, placing it in seventh place , and Italy, with 22.4 million, as number eight, as well as Japan, with 21.1 million, and Russia, with 20.9 million.

SPOTLIGHT ON IMMUNIZATION

The Centers for Disease Control and Prevention said that, since last Thursday, more than 263.8 million people in the United States – or 79.5% – have received at least one dose of the coronavirus vaccine. Of that population, 67.8%, or 224.9 million people, have received two doses of vaccine, and the total number of doses that have been distributed in the United States is now 616.2 million. Breaking it down further, 90.4% of the population over 18 – or 233.4 million people – have received at least one first vaccination and 77.5% of the same group – or 200.1 million people – are fully vaccinated. In addition, 51.8% of this population, or 103.7 million people, have already received a first booster dose of vaccine.

Beginning June 13, 2022, the CDC began updating vaccine data on a weekly basis and releasing updated information on Thursdays at 8 p.m. EDT, according to a statement posted on the CDC’s website. agency.

Some 68% of the world’s population received at least one dose of the coronavirus vaccine on Wednesday, according to Our World in Data, an online science publication that tracks such information. So far, 12.72 billion doses of the vaccine have been administered worldwide and 3.22 million doses are now being administered every day.

Meanwhile, only 22.6% of people in low-income countries have received a dose, while in countries like Canada, China, Denmark, France, Italy, the UK and the United States, at least 75% of the population has received at least one dose of the vaccine.

Only a few of the world’s poorest countries – Bangladesh, Bhutan, Cambodia and Nepal – have reached the 70% vaccination mark. Many countries, however, are below 20%, and in countries like Haiti, Senegal and Tanzania, for example, vaccination rates remain in the single digits or even lower.

Moreover, with the start of vaccinations in North Korea, Eritrea remains the only country in the world that has not administered vaccines.

Anna Breuer contributed reporting for this story.

(Photo: Accura Media Group)

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‘My Alcohol Diary’: Crush Reveals He Hated Playing THAT OST People Love https://nsms10.com/my-alcohol-diary-crush-reveals-he-hated-playing-that-ost-people-love/ Sat, 24 Sep 2022 11:46:37 +0000 https://nsms10.com/my-alcohol-diary-crush-reveals-he-hated-playing-that-ost-people-love/ On a recent episode of Lee Young Ji’s “My Alcohol Diary,” Crush confessed that he doesn’t want to perform a certain OST sung by him on stage, even though a lot of people like this song. Keep reading to find out which track it is. ‘My Alcohol Diary’: Crush Reveals He Hated Playing THAT OST […]]]>

On a recent episode of Lee Young Ji’s “My Alcohol Diary,” Crush confessed that he doesn’t want to perform a certain OST sung by him on stage, even though a lot of people like this song. Keep reading to find out which track it is.

‘My Alcohol Diary’: Crush Reveals He Hated Playing THAT OST People Love

On September 23, Crush took part in the latest episode of Lee Young Ji’s “My Alcohol Diary” series (also known as “No Prepare”). At times, the conversations turned serious as the R&B singer gave Lee advice on music and shared a story about when he was at his lowest.

(Picture: Instagram: @crush9244)
‘My Alcohol Diary’: Crush Reveals He Hated Playing THAT OST People Love

Lee Young Ji got his start as a rapper and initially wanted to focus on producing rap-centric music. But now she wonders if she should also include singing or dancing in her performances.

On this subject, she asked Crush how to seek her musical direction. The male artist replied that she should think of creating music like writing a diary.

ALSO READ: EXO DO and Crush Melt Hearts with their rendition of “Beautiful” at Park Shin Hye and Choi Tae Joon’s wedding

Crush on my liquor log

(Picture: YouTube: 차린건 쥐뿔도 없지만)
‘My Alcohol Diary’: Crush Reveals He Hated Playing THAT OST People Love

The male idol also confessed that he himself struggled with his musical direction, especially back when he was at one of the most successful times in his career, i.e. say when he released the hit OST “Beautiful.”

As a reminder, Crush’s “Beautiful” served as the OST for popular tvN drama “Goblin.” The song quickly reached No. 1 on national music charts such as MelOn, Mnet, Bugs, Olleh Music, Genie, Monkey3, and Naver Music shortly after its release on December 31, 2016.

Crush the beautiful

(Picture: Twitter: @MyKoreanDramaID)
‘My Alcohol Diary’: Crush Reveals He Hated Playing THAT OST People Love

Returning to the latest episode of Lee Young Ji’s “My Alcohol Diary,” Crush said:

“The time I suffered the most from a slump was when I released ‘Beautiful’ five years ago. That song got a lot of love from people. very grateful but on one side I felt empty.”

The R&B singer went on to say that he was grateful that people liked the song and that he was repeatedly asked to sing it at weddings. However, he revealed that he actually didn’t want to perform this OST on stage.

Crush on my liquor log

(Picture: YouTube: 차린건 쥐뿔도 없지만)
‘My Alcohol Diary’: Crush Reveals He Hated Playing THAT OST People Love

READ MORE: Red Velvet Joy Silent Cheers for Boyfriend Crush – Here’s how

Crush explained to Lee Young Ji, “Because this song was a bit different from the song I chased so far.”

The “Rush Hour” singer used to work with those in Hongdae underground, like Beenzino, Zion.T, Swings and Giri Boy, and this hip-hop/R&B direction was the only genre he wanted. continue at the time. However, “Beautiful” was a ballad.

Crush on my liquor log

(Picture: YouTube: 차린건 쥐뿔도 없지만)
‘My Alcohol Diary’: Crush Reveals He Hated Playing THAT OST People Love

At that time, he was musically puzzled that he would end up having panic attacks, which made it difficult for him to sing the OST on stage. In fact, there was a time when he was unable to breathe.

Crush on my liquor log

(Picture: YouTube: 차린건 쥐뿔도 없지만)
‘My Alcohol Diary’: Crush Reveals He Hated Playing THAT OST People Love

But Crush also clarified that was then and now he can sing “Beautiful” ten times in a row if asked.

Do you think Crush should release more ballads in the future?

Crush’s ‘Rush Hour’ Featuring J-Hope Tops iTunes Charts Around the World

In other news, Crush made his first comeback since his military discharge last August on September 22. The R&B singer has released his new digital single “Rush Hour” featuring BTS J-Hope.

YOU MIGHT ALSO LIKE: Crush reveals how he ended up collaborating with J-Hope for ‘Rush Hour’

Rush hour JHope

(Photo: Twitter: @OfficialPnation)
‘My Alcohol Diary’: Crush Reveals He Hated Playing THAT OST People Love

As of the morning of the 23rd, “Rush Hour” has reached No. 1 on the iTunes Song Charts in at least 41 different countries, including Chile, Mexico, Brazil, the Philippines, and Indonesia.

The digital single even landed in the top 10 of 68 other regions, including the United States (where it took No. 2), Japan (No. 3) and the United Kingdom (No. 4).

Check out Crush’s “Rush Hour” music video featuring J-Hope below:

For more K-Pop news and updates, keep your tabs open here on KpopStarz.

KpopStarz owns this item

Written by Maria Scott

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Australia lose to France in opener, Lauren Jackson returns, stats, highlights https://nsms10.com/australia-lose-to-france-in-opener-lauren-jackson-returns-stats-highlights/ Thu, 22 Sep 2022 12:33:00 +0000 https://nsms10.com/australia-lose-to-france-in-opener-lauren-jackson-returns-stats-highlights/ Australia’s World Cup campaign suffered a major setback after a disappointing 70-57 loss to France in a forgettable opener. The Opals were desperate to usher in a bold new era on home soil, but they couldn’t contain a powerful French side led by Seattle Storm star striker Gabby Williams. Australia couldn’t deny Williams, who dominated […]]]>

Australia’s World Cup campaign suffered a major setback after a disappointing 70-57 loss to France in a forgettable opener.

The Opals were desperate to usher in a bold new era on home soil, but they couldn’t contain a powerful French side led by Seattle Storm star striker Gabby Williams.

Australia couldn’t deny Williams, who dominated the proceedings with her athleticism to finish with a game-high 23 points.

Watch every game of the FIBA ​​Women’s Basketball World Cup 2022 live and free on ESPN with Kayo Freebies. Register now, no credit card required.

Opals guard Bec Allen did his best to keep Australia in the game, dropping a team-high 16 points.

In the end, however, Australia lacked the firepower of France.

The Opals now need to win at least three of their last four games against Mali, Serbia, Canada and Japan to qualify for the quarter-finals.

It won’t be an easy task, especially with tough tests against Canada and Japan.

JACKSON BELONGS BUT DIDN’T DOMINATE

It’s no coincidence that Jackson looked comfortable without making a major impact in his first international game for the Opals since 2013.

Spies tell me Australia’s greatest basketball player was already training for a comeback last May, about 500 days away from the World Cup.

Jackson had also given up alcohol, so she was fully determined to don green and gold again.

That discipline and dedication showed in Lauren’s performance against France.

Sure, she’s not the Jackson of old and she only scored three points and added a rebound in 10 minutes on the floor, but she can still make an impact, even at 41 years old.

The tall Australian came into the game with five minutes left in the first quarter.

She missed her first three-point attempt, but she didn’t mind.

Jackson quickly moved on to a crucial flight.

It gave him the confidence to come back to the three-point line and empty a dagger, bringing the strong Sydney SuperDome crowd to their feet.

Watch for Jackson’s improvement as the tournament progresses.

She remained positive after the game, saying, “It’s a long tournament and you can bounce back.”

Jackson missed the entire third quarter as France extended their lead but said after the game she was “fine”.

“I made two mistakes, but I’m fine (physically),” she said.

“I just do what I’m told.”

ALLEN EXCELS, BUT NEEDS MORE HELP

The Opals looked sluggish in attack at some stages, but Allen provided the Australians with much-needed stability.

The gun guard went after the game in the first half with some aggressive shooting.

The New York Liberty WNBA star had 11 points in the first half to keep the Opals in the contest.

France led by one point at the quarter and half time, but the Australians refused to start again.

Allen continued to dominate in the second half, but she needs more help from the attacking side.

WILLIAMS PUT FEAR THROUGH OPALS

France were understaffed, but that didn’t matter as Williams put on a show.

The French have fielded a team of young lesser big names – guards Marine Johannes (thigh), Bria Hartley and center Sandrine Gruda.

Johannes, ranked best player in France, was particularly a loss for France.

It was feared the French might struggle without their star trio, but Williams was brilliant.

The Seattle Storm WNBA star did not play in France’s latest loss to Australia earlier in the week.

Opals captain Tess Madgen was suspicious of Williams before the game.

“Gabby didn’t play in our warm-up game so we’ll have to adapt to his athleticism,” Madgen said before the game.

The Opals skipper was in the money as Williams produced a better performance on the pitch for the Frenchman.

Australia must quickly regroup ahead of Friday’s must-see game against Mali from 8:30 p.m. (AEST).

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Carro expands into Japan through partnership with Softbank https://nsms10.com/carro-expands-into-japan-through-partnership-with-softbank/ Tue, 20 Sep 2022 06:00:33 +0000 https://nsms10.com/carro-expands-into-japan-through-partnership-with-softbank/ Singapore-based used-car market Carro on Tuesday announced its expansion into Japan through a joint venture with SoftBank Corporation (SoftBank). As part of its entry, CARRO JAPAN Co., Ltd. (CARRO JAPAN) aims to introduce its full range of services, such as subscription services and car rental, Carro said in a statement. Through this strategic partnership, Carro’s […]]]>

Singapore-based used-car market Carro on Tuesday announced its expansion into Japan through a joint venture with SoftBank Corporation (SoftBank).

As part of its entry, CARRO JAPAN Co., Ltd. (CARRO JAPAN) aims to introduce its full range of services, such as subscription services and car rental, Carro said in a statement.

Through this strategic partnership, Carro’s technology will digitally match traditional car leasing to business users in Japan. The partnership will also involve several leasing companies, with plans to expand the market to individual users.

“Japan is one of the largest automotive markets and one of the most digitally advanced countries in Asia. Our entry into Japan is the natural next step to increase our regional presence and help traditional dealerships expand their market presence across the country using our capabilities based on Artificial Intelligence (AI) technology,

“Over the next few years, we plan to capitalize on the growth of the used car market. We will start by providing car subscription services with Softbank initially, and eventually plan to work with thousands of dealerships across Japan to play an active role in providing consumers with a seamless buying and selling experience. hassle,” said Carro CEO and co-founder Aaron Tan.

According to the statement, Japan has seen an increase in demand for used cars and subscription services in recent years, resulting from rising prices for new vehicles and a growing need to own a car.

However, the difficulty in accurately assessing the valuation of a used car due to the inconsistency and lack of credible valuation criteria has led to several barriers to entry into the used car market. opportunity in the country.

Carro said CARRO JAPAN will support existing car dealerships with leasing services and provide a highly competitive subscription service.

Additionally, Carro’s AI technology will be integrated to accurately predict the valuation of used cars based on their history and data.

Meanwhile, SoftBank’s key role in CARRO JAPAN will be to tailor services to the Japanese market, including marketing, sales and collaboration with partner companies.

With the entry of Carro in Japan, corporate customers can apply for the subscription service by indicating their requirements for a used car through the CARRO JAPAN website, including vehicle size, mileage, non- smoker or smoker, and more.

A list of suitable used cars available for hire will automatically be generated for review.

In addition, CARRO JAPAN will also allow users to request various auxiliary services that will improve the efficiency and safety of operations related to the use and management of company-owned vehicles.

Available services will include a vehicle management solution, which records driving habits such as acceleration and deceleration, as well as an alcohol detection function to promote safe driving.

Other features, such as smart keys, will allow users to unlock and lock their vehicles using their smartphones.

CARRO JAPAN plans to extend this service to individual users by collaborating with LINE Corporation, a SoftBank Group company.

Under the joint venture, SoftBank will lead its “Beyond Japan” initiative to expand into East Asian countries and promote business development outside of Japan.

Founded in 2015, Carro is Southeast Asia’s largest online car retailer. The company transforms the traditional way of buying and selling cars through proprietary pricing algorithms, AI-enabled capabilities and innovative technology solutions.

Based in Singapore, the company has a strong presence in Asia Pacific, supported by more than 2,000 employees in Indonesia, Thailand, Malaysia and Vietnam.

The company has raised over 700 million Singapore dollars ($497 million) from the Softbank Vision Fund and several sovereign wealth funds.

Singapore’s Carro achieves profitability in FY22 as revenue more than doubles

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Osteogenesis in human periodontal ligament stem cell sheets is enhanced by protease-activated receptor 1 (PAR1) in vivo https://nsms10.com/osteogenesis-in-human-periodontal-ligament-stem-cell-sheets-is-enhanced-by-protease-activated-receptor-1-par1-in-vivo/ Sun, 18 Sep 2022 12:28:23 +0000 https://nsms10.com/osteogenesis-in-human-periodontal-ligament-stem-cell-sheets-is-enhanced-by-protease-activated-receptor-1-par1-in-vivo/ We confirm that all methods have been carried out in accordance with ARRIVE guidelines and relevant guidelines and regulations. Ethics statement Informed consent was obtained from all subjects and ethics committee approval from the Ethics Committee Review Board of the University of São Paulo School of Dentistry (protocol number FO-USP 029/2018) was obtained prior to […]]]>

We confirm that all methods have been carried out in accordance with ARRIVE guidelines and relevant guidelines and regulations.

Ethics statement

Informed consent was obtained from all subjects and ethics committee approval from the Ethics Committee Review Board of the University of São Paulo School of Dentistry (protocol number FO-USP 029/2018) was obtained prior to collection of the patient’s teeth at the hospital. clinic of the School of Dentistry of the University of São Paulo (FO-USP). The use of Balb/c nude mice in this research has also been approved by the Board of Directors of the Ethics Committee on the Use of Animals (CEUA) of the Institute of Chemistry of the University of Sao Paulo ( IQ-USP) under protocol number 98/2018.

Cell isolation

Periodontal ligament stem cells were harvested from third molars of 3 systemically healthy individuals (I1, I2, and I3—18-30 years old). The inclusion criteria were: partially or totally erupting third molars and absence of periodontal disease.

Periodontal ligament tissue samples were obtained by scaling the middle third of the root and PDLSCs were isolated using the explant technique12.48 in alpha-modified Eagle’s medium (α-MEM) (Thermo Fisher Scientific, Waltham, USA) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific, Waltham, USA), 100 μg/mL penicillin , 100 μg/mL streptomycin and 0.5 mg/mL amphotericin B (Gibco, Invitrogen, Carlsbad, USA) at 37°C in an atmosphere of 5% CO2 and 95% humidityseven. After 14 days, cells from the explants reached 70% confluence and PDLSC populations were used at passage 4 for all experiments.

Characterization of pluripotency

In order to identify the mesenchymal stem cell phenotype, approximately 5 × 105 PDLSCs were incubated in 5% bovine serum albumin (BSA)/phosphate buffered saline (PBS) (Gibco, Invitrogen, Carlsbad, USA) 1 × at 4°C in the dark for 1 h with the following monoclonal antibodies: PAR1-FITC (Abcam, Cambridge, UK), OCT4-FITC (Abcam, Cambridge, UK), SOX2-FITC (Abcam, Cambridge, UK), STRO-1-FITC (Abcam, Cambridge, UK Uni), CD14-FITC (eBioscience, San Diego, USA), CD90-FITC (eBioscience, San Diego, USA), CD31-PE (eBioscience, San Diego, USA), CD-44-PE (eBioscience, San Diego, USA), CD34-FITC (Biolegend, San Diego, USA) and CD146-PE (Biolegend, San Diego, USA) for 30 min at 4°C. An uncolored witness was used to define the gates. A total of 10-50,000 events were recorded and the data analyzed via FlowJo (Becton Dickinson, Oregon, USA).

Cell sheet culture and experimental design

PDLSC at 1×106 cells/cm2 were seeded in 100 mm plates for 24 h with α-MEM (Thermo Fisher Scientific, Waltham, USA) supplemented with 10% FBS (Thermo Fisher Scientific, Waltham, USA) and 50 μg/mL vitamin C to induce cell sheet formation, as previously describedseven. Subsequently, cell sheets were assigned to one of the following experimental groups: (1) control medium (CTRL) composed of α-MEM (Thermo Fisher Scientific, Waltham, USA) supplemented with 10% FBS (Thermo Fisher Scientific, Waltham, USA) and 50 μg/mL vitamin C (Sigma-Aldrich, St Louis, USA); (2) osteogenic medium (OST) (CTRL + 100 nM dexamethasone, 5 mM b-glycerophosphate and 50 µg/ml ascorbic acid (Sigma-Aldrich, St Louis, USA) and (3) osteogenic medium with the addition of BY1 agonist peptide (100 nM TFLLR-NH2) (Tocris Bioscience Inc., Bristol, UK) (OST + PAR1). The culture medium was changed every 3 days for 14 days.

Alizarin red staining

I2 PDLSCs were seeded in 6-well plates (5 × 104 cells/cm2), in triplicate. After processing with CTRL, OST and OST + PAR1 groups for 14 days, cell sheets were washed with 1 × PBS (Sigma-Aldrich, St Louis, USA), fixed with 4% paraformaldehyde (Sigma-Aldrich, St Louis, USA) for 15 min at room temperature ( TA), washed with 1 × PBS (Sigma-Aldrich, St Louis, USA) and incubated with a stirred solution of 2% Alizarin Red S (Sigma-Aldrich, St Louis, USA) in PBS (Sigma-Aldrich, St Louis , USA) (pH 4.2) (A5533) for 30 min at RT. For qualitative and macroscopic analysis, images were acquired using a CDD microscope camera (D7000, Nikon, Minato, Japan). For quantitative analysis, 10% ammonium hydroxide solution (Sigma-Aldrich, St Louis, USA) was used to dilute the calcium deposits and a spectrophotometer (Biotek, Winooski, USA) was used to measure the absorbance at 405 nm. The quantification of ARS was calculated using a standard curve.

Characterization of the cell sheet

Scanning electron microscopy

Cell sheets at 14 days of treatment were fixed with Karnovsky’s solution (Thermo Fisher Scientific, Waltham, USA) for 24 h. After this period, post-fixation was performed with osmium tetroxide (Sigma-Aldrich, St Louis, USA) for 2 h and dehydration, passing the material through an increasing series of alcohol (Sigma-Aldrich, St. Louis, USA) from 70 to 100%. The samples were then dried under critical point conditions and sputtered gold coated. Observations were then made using a scanning electron microscope (S-4800; Hitachi, Tokyo, Japan).

Histological profile

Hematoxylin and eosin (Sigma-Aldrich, St Louis, USA) staining of cell sheets was also performed. Briefly, after detachment and fixation in 4% paraformaldehyde (Sigma-Aldrich, St Louis, USA) for 24 h, specimens were embedded in paraffin and sectioned at 4 µm thickness. After the sectioning process, the histological specimens were placed in a 50°C water bath and captured on a histological slide and stored in a 65°C oven for 12 h to allow removal of paraffin remnants. Sequentially, the paraffin removal dehydration protocol was completed using a sequence of solutions of xylol (Sigma-Aldrich, St Louis, USA) and alcohol (Sigma-Aldrich, St Louis, USA) and specimens underwent routine H&E staining according to manufacturer’s guidelines.

Ectopic bone formation assays

Bone formation in vivo was assessed using transplantation of cell sheets pre-incubated with the experimental groups described above. At 14 days of incubation, the cell sheets were detached using a cell scraper (Corning, New York, USA), folded 4 times to acquire a cylindrical shape and each Balb/c nude mouse aged 6 weeks received 2 back grafts from the same experiment. group, bilaterally. A total of 25 mice were divided into the groups as follows: 11 (OST + PAR1), 11 (OST) and 3 (CTRL). After 60 days, mice were euthanized and subcutaneous samples were collected and processed for analysis described below.

Computerized microtomography

After fixation, only the subcutaneous sample from the left side of each animal was scanned using a micro-CT system (Skyscan 1176, Bruker Biospin, Billerica, USA) with the following acquisition parameters: 45 kV, 8.71 μm resolution and 550 μA. The data obtained were processed, reconstructed into three-dimensional images using reconstruction software (NRecon, Micro Photonics, Allentown, USA) and analyzed using system software (CTAn, Bruker Biospin, Billerica, USA).

Immunohistochemistry and histological analysis

Histological procedures were performed as previously described. H&E, Trichrome de Masson, Van Gieson and Von Kossa stains (Sigma-Aldrich, St Louis, USA) were performed according to the manufacturer’s protocol. For immunohistochemical analysis, after fixation in a 4% paraformaldehyde solution in PBS for 30 min, samples were embedded in paraffin and sectioned at 20 µm. Sections were subjected to deparaffinization, rehydration and permeabilized with 0.2% Triton X-100 (Sigma-Aldrich, St Louis, USA) for 10 min. Next, sections were blocked in 3% hydrogen peroxide solution (Sigma-Aldrich, St Louis, USA) for 10 min, followed by antigen retrieval with 10% citrate buffer ( Sigma-Aldrich, St Louis, USA) for 15 min and blocking in 5% BSA (Sigma-Aldrich, St Louis, USA) for another 30 min. Then, exposure to specific primary antibodies was performed for: integrin β1 – AB52971 (Abcam, Cambridge, UK), type I collagen – AB34710 (Abcam, Cambridge, UK) and BSP – AB8448 (Abcam, Cambridge, UK). Slides were then incubated with secondary conjugated anti-rabbit IgG antibody – AB205718 (Abcam, Cambridge, UK) followed by incubation of avidin-biotin complex (Abcam, Cambridge, UK) for 30 min. . Diaminobenzidine (DAB) solution (Abcam, Cambridge, UK) was applied every 2 min and the reaction observed under the microscope. Specimens were dehydrated, cleaned and mounted. Images of the specimens were obtained with an optical microscope (E600, Nikon, Tokyo, Japan) and three images of each sample were acquired and quantified using the J-frame (NIH, Bethesda, USA).

statistical analyzes

Experiments were performed in triplicate and data were expressed as mean ± standard deviation (SD). Data were analyzed based on the comparison between the experimental group (OST + PAR1) with osteogenic control group (OST) and control group (CTRL). A one-way ANOVA followed by Tukey’s post-hoc test was used. A meaning P-value of 0.05 was established for all tests and data analysis was performed (GraphPad Prism™ Version 6.0c, La Jolla, USA).

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4 gourmet autumn finds to offer https://nsms10.com/4-gourmet-autumn-finds-to-offer/ Fri, 16 Sep 2022 22:05:38 +0000 https://nsms10.com/4-gourmet-autumn-finds-to-offer/ Some reports indicate that, under current economic conditions, holiday shopping is set to happen much earlier this year, well before the usual bonanza of “Black Friday” and “Cyber ​​Monday” in November. It looks like fall shopping has gotten a lot bigger this year. While the season always ushers in an exciting crop of innovative and […]]]>

Some reports indicate that, under current economic conditions, holiday shopping is set to happen much earlier this year, well before the usual bonanza of “Black Friday” and “Cyber ​​Monday” in November. It looks like fall shopping has gotten a lot bigger this year. While the season always ushers in an exciting crop of innovative and decidedly wallet-worthy solutions, this year our fall product picks pay particular attention to those that can do double duty, both pleasing the buyer and be well suited to offer to others. Below are some great ideas.

Colsen Portable Fireplaces (https://colsenfirepits.com/)
S’mores anyone? Enjoy the ambiance of a real smokeless fire and clean up with Colsen Fire Pits, a portable, eco-friendly solution that’s easy to light and extinguish. Unlike traditional fireplaces, Colsen specializes in enjoyment on the inside for year-round enjoyment. They are designed to work in any environment, so customers can also enjoy them outdoors. The brand recommends using 70% or 91% isopropyl alcohol as fuel for a clean burn that won’t leave soot on your ceiling. Made from temperature resistant concrete, Colsen fire pits are made with high quality materials that create an enhanced, safe, smoke and odor free experience that is meant to last a lifetime. Modern, portable designs provide an easy way to set the stage for an inviting atmosphere for quiet times alone or while celebrating with loved ones. Use it to make the perfect batch of S’mores or just to enjoy over 40 minutes of cozy fire time. Homeowners can even use their Colsen fireplaces for two purposes by adding essential oils to the top or mixing lemongrass oil with alcohol to ward off unwanted bugs. All packages include a cork or bamboo fire extinguisher cover, fire pit and an instruction card which includes guidelines for 100% safety when lighting and extinguishing the item. Shoppers can choose from seven shapes, including rectangle, round, square, large hex, small hex, tesla hex and more.

4 gourmet autumn finds to offer


Tropical and Exotic Fruit and Root Boxes by Tropical Fruit Box (
https://tropicalfruitbox.com/)
For a healthy sweet treat, check out Tropical Fruit Box, a company that sources fresh, tropical and exotic fruits from around the world and delivers them right to customers’ doorsteps. Stocked with some of the hardest-to-find fruits and roots that are often not found in local grocery stores (and certainly not with this level of quality and freshness), Tropical Fruit Box transports customers to new or nostalgic destinations through a mixture of tastes and aromas. This women-led company partners with family farms in Florida, South America, Central America, the Caribbean, and tropical regions of Africa and Asia to provide ripe, non-GMO, and fresh produce. optimal freshness. Buyers can choose from single fruit boxes and customer favorite variety options like Taste the Tropics, Taste the Exotics, Tropi Travel or the exclusive “Create Your Own” boxes which feature unique fruit selections that also offer a immune boosting experience. Tropical Fruit Box is also part of a group of exclusive online retailers of the new PinkGlow pink pineapple, which comes in its own special gift box. Succulent, sweet and juicy fruits are sustainably sourced and grown on a carbon neutral farm in Costa Rica. Here’s a fun fact if chocolate is your thing: it’s made from the fruit of the cacao tree. Chocolate lovers can take their passion and culinary prowess even further with the new The Chocolatier’s Apprentice Box, which provides customers with cocoa pods, silicone flower molds and a step-by-step guide on how to make your own chocolate. Plus, all Tropical Fruit Box gifts come with fruit reference cards and chef-inspired recipes, making these boxes a fruitful gift for health connoisseurs and home chefs alike.

4 gourmet autumn finds to offer

MUJEN Spirits (https://mujen.com)
Created by longtime friends Sondra Baker and Bruce Bozzi, MUJEN is the best spirit you’ve probably ever tasted. This new line of healthier premium spirits is gluten-free, carb-free and sugar-free. Made from the purest ingredients – water, rice and koji – MUJEN is a type of distilled spirit called shochu, considered the number one spirit in Japan with a rich history dating back over 500 years. Baker and Bozzi’s journey to bring the ancient drink to a modern palate began with the couple’s trip to Japan’s most esteemed distilleries. There they came across Sengetsu in Kumamoto Prefecture, a family-run Japanese distillery that has been making shochu for more than four generations. Their partnership resulted in the MUJEN brand, a play on the Japanese word for infinity, “mugen”, which comes in three different varieties, each expertly crafted to serve discerning audiences. MUJEN Original slips perfectly into your favorite cocktail as an alternative to vodka, tequila, gin or rum, while MUJEN Ai Lite offers fewer calories and less alcohol. Finally, MUJEN X is a 10-year-old barrel-aged reserve that is best enjoyed neat or over ice, like a fine whiskey or tequila añejo. The profile of Shochu is smooth, silky and is often compared to the taste of single malt Scotch or Japanese whiskey. Additionally, its drinkers claim to have fewer hangovers due to the alcohol containing less alcohol and additives than traditional competitors. At the heart of MUJEN, however, are the special celebrations Baker and Bozzi hope to share around the drink. Each installment is meant to be a toast to bonding, having fun, and enjoying life’s little moments.

4 gourmet autumn finds to offer

Truffoire White Truffle Face Peeling Treatment (https://www.truffoireworld.com)
Who said truffles were just for eating? Truffoire White Truffle Facial Scrub is specially formulated with minerals, vitamins, antioxidants and white truffle extract to provide skin with deep cleansing, complete hydration and hydration. Most facial peels on the market focus on one thing: cleansing, and many just clean the surface. While this is certainly essential, it is not enough for a completely refreshed skin look and feel. Powerful facial cleanser gently fades the look of built-up dry skin caused by pollution, dirt, sun exposure and makeup residue. The result will leave you looking more supple and prevent UV damage and the formation of free radicals, while fighting wrinkles and uneven skin tone and texture. This helps give your skin a refreshed and new appearance. The company’s award-winning formula is a best-seller with five-star reviews on its website. Key ingredients include white truffle for its anti-aging properties, green tea leaf extract with powerful antioxidants, bamboo powder rich in minerals and proteins which hydrate, vitamin C which stimulates collagen and retinol (vitamin A) To protect the skin from UV damage. .

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Merilee Kern, MBA is an internationally renowned brand strategist and analyst who reports on cultural shifts and trends as well as notable industry players, doers, shakers and innovators across all categories, both B2C and B2B. This includes field experts and thought leaders, brands, products, services, destinations and events. Merilee is the founder, editor and producer of “The Luxe List” as well as the host of “Wise living lifestyle television show airing in New York, Los Angeles, San Francisco, Miami, Atlanta and other major markets on CBS, FOX and other major networks; as good as “Wise companies“Commercial television broadcast nationally on FOX Business TV and Bloomberg TV. As a prolific spokesperson for the lifestyle, travel, dining and leisure industry, Merilee keeps her finger on the pulse of the market in search of new, innovative must-haves and exemplary experiences at every turn. prices, from affordable to extreme. . Her work reaches several million people worldwide via broadcast television (her own shows and many others on which she appears) as well as a myriad of print and online publications. Connect with her on www.TheLuxeList.com and www.SavvyLiving.tv / Instagram www.Instagram.com/MerileeKern /Twitter www.Twitter.com/MerileeKern / Facebook www.Facebook.com/MerileeKernOfficial /LinkedIN www.LinkedIn.com/in/MerileeKern.

Some or all of the accommodations, experiences, features and/or services detailed above may have been provided or arranged at no cost to fit if this is an editorial, but all opinions expressed are entirely those of Merilee Kern and were not influenced.

Source:

Inflation May Lead to Early Christmas Shopping

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Jim Beam plans $400 million expansion in Kentucky Bourbon https://nsms10.com/jim-beam-plans-400-million-expansion-in-kentucky-bourbon/ Thu, 15 Sep 2022 01:00:36 +0000 https://nsms10.com/jim-beam-plans-400-million-expansion-in-kentucky-bourbon/ For sharper, more insightful business and economic news, subscribe to The Daily Upside newsletter. It’s completely free and we guarantee you’ll learn something new every day. The company famous for its brown water wants to be green – and it’s spending big bucks to get there. To meet growing demand, Jim Beam maker Beam Suntory […]]]>

For sharper, more insightful business and economic news, subscribe to The Daily Upside newsletter. It’s completely free and we guarantee you’ll learn something new every day.

The company famous for its brown water wants to be green – and it’s spending big bucks to get there.

To meet growing demand, Jim Beam maker Beam Suntory said Wednesday it will roll out a $400 million expansion at its largest Kentucky distillery and power it with renewable energy.

Two birds with a whiskey stone

Beam Suntory, which also makes the fancier Maker’s Mark craft whiskey and is owned by Japan’s Suntory, has a two-part environmental plan. First, to halve greenhouse gas emissions and water consumption by 2030. Second, to remove more carbon from the atmosphere than its activities emit by 2040, by part by planting more than 500,000 trees every year for the rest of the decade, or enough to offset the ones he needs to make casks for aging whisky.

With the Jim Beam factory in Boston, Kentucky* running out of capacity (no New England non-rhoticity here, it’s all Southern twang), the time has come for a green project that also allows the company to make a lot more of the most bourbon the most sold to the world:

  • The new expansion will increase capacity at the Boston plant by 50% and produce more Jim Beam White Label and Black Label bourbons, most of which will support anticipated sales growth in Europe and Asia. Beam Suntory reported global sales growth of 11% last year, with double-digit growth in China and India.
  • When the new project is completed in 2024, the distillery will derive 65% of its electricity from renewable natural gas and 35% from fossil-based natural gas. The trick here will be the construction of a new facility across the street, in partnership with renewable energy developer 3 Rivers Energy Partners, which will convert waste from bourbon manufacturing into biogas.

Proof of concept: About 95% of the world’s bourbon production is based in Kentucky, forming the heart of the state’s $9 billion distilling industry. According to the Kentucky Distillers’ Association, distillers are planning $5 billion in capital investment. Alcohol has generally been a recession-proof industry – and the most resilient products are doing particularly well. According to the Cowen researchers, distillers now hold 41.5% of the alcohol market in the United States, with much of this growth coming at the expense of breweries, thanks to their luxury status, cocktail culture and their health and wellness trends. Let’s say it cheers them up.

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What happened when a kamikaze pilot returned? https://nsms10.com/what-happened-when-a-kamikaze-pilot-returned/ Mon, 12 Sep 2022 17:00:23 +0000 https://nsms10.com/what-happened-when-a-kamikaze-pilot-returned/ The Japanese use of kamikaze pilots in the Pacific campaign against the United States was perhaps the most extreme tactic ever used by the Empire of the Rising Sun to gain an advantage over the enemy. This tactic grew out of the need to destroy American aircraft carriers, which defined American dominance in the Pacific […]]]>

The Japanese use of kamikaze pilots in the Pacific campaign against the United States was perhaps the most extreme tactic ever used by the Empire of the Rising Sun to gain an advantage over the enemy. This tactic grew out of the need to destroy American aircraft carriers, which defined American dominance in the Pacific theater. American carriers were usually heavily guarded by both high quality American fighters and anti-aircraft guns mounted on the carrier itself.

During the later stages of the war, Japanese fighter aircraft could not hope to compete with superior American variants and so a new way to strike at the heart of the American fleet was needed. These circumstances gave rise to the kamikaze tactic. But what if a pilot tasked with this vital task fails his mission and returns?

for the emperor

Honor has always played a big role in Japanese society. Many who volunteered in the Kamikaze Corps saw it as an honor to die for their country. It was also believed that the show of honor and selflessness of a kamikaze pilot voluntarily sacrificing himself for this country would have massive psychological impacts on the invading forces.

More importantly, kamikaze attacks were far more precise than bombardment, allowing the Japanese air force to target weak points in American ships, making this tactic much more effective than traditional bombardment, something vital to the already weak and overstretched Japanese army. forces.

The USS Bunker Hill was hit by suicide bombers piloted by Ensign Kiyoshi Ogawa (pictured below) and Lt. Junior Grade Seizō Yasunori on May 11, 1945. Of a crew of 2,600 at Bunker Hill, 389 were killed. were killed or officially missing, and 264 were injured. Source: Wikimedia Commons

Many people in Japanese society admired kamikaze pilots as they were seen as the ultimate proof of loyalty to the emperor. As such, they received better rations during their training, though this was offset by the harsh training and discipline they underwent while preparing for their final day. Even with all this preparation and nationalist zeal, some of the pilots were too scared to complete their task or experienced mechanical failures on their “last flight”, forcing them to return to Japan.

Failure and dishonor

Kamikaze pilots with a puppy showing us the humanity of these unfortunate souls. Source: Wikimedia Commons

The returning kamikaze pilots fall into two distinct groups. Those who returned due to weather conditions or mechanical breakdowns in their place and those who returned because they could not complete their task successfully due to psychological reasons. Each group received a different treatment upon their return.

Pilots who could prove that their return was caused by conditions beyond their control were not punished or looked down upon. At a stage in the war when even pilots were considered a scarce resource, the Japanese could not afford to lose a well-trained kamikaze pilot; thus, their return was accepted and their “last flight” rescheduled, though some still feel survivor’s guilt if they were the only ones in their squadron to survive.

For pilots who could not prove that their return was caused by factors beyond their control, their treatment would be a little different. Although still not executed, these pilots would receive some sort of punishment, whether physical or mental. These punishments were not to be too severe as the pilot had to be ready for another flight at some point in the future, so nothing that would impair this ability would be done. Even so, it had a limit, as seen with a pilot who returned 9 times from his Kamikaze mission. He will be executed on his 9th return for cowardice.

To combat these mental factors that would prevent kamikaze pilots from performing their duties, a few measures were put in place. Kamikaze pilots often flew in squadrons to increase peer pressure between colleagues, which reduced the number of pilots who collapsed and failed to complete their task. The pilots were also given alcohol before their “last flight”, which gave them “liquid courage” to help them complete their task.

The squadron of Kamikaze pilots take their last drink before their mission. Source: Wikimedia Commons

Many believe the pilots were also given just enough fuel to complete their goal as another incentive, but that wasn’t true as we now know these pilots were such a scarce resource that it wouldn’t be a viable way to incentivize. . them to do their job.

When you eliminate all thoughts of life and death, you can totally ignore your earthly life. It will also allow you to focus your attention on eradicating the enemy with unwavering determination, while building up your excellence in flying skills. — Excerpt from a kamikaze pilot’s manual

Despair

As always, war brings out the worst in humanity, which couldn’t be more true of the Japanese. In a society where honor and submission to the emperor mean more than life, tactics such as the use of kamikaze pilots or the manufacture of suicide bomb infantry tanks could be justified and even celebrated by the general population because they helped the war effort.

Japan would commit many despicable war crimes during World War II in an attempt to defeat its enemies, without stopping anything to do so. We see this with the experiments carried out by Unit 731 and the atrocities committed in mainland China in a quest to appease the rebellious population.

Japan has always been an interesting case study in history and will remain so due to the unique culture the hermit civilization has developed over centuries of isolation from influence outside of their island. I look forward to exploring more of the history of this country one day.

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Josh Freed: Two teaspoons of wine might be a little too much moderation https://nsms10.com/josh-freed-two-teaspoons-of-wine-might-be-a-little-too-much-moderation/ Sat, 10 Sep 2022 10:02:10 +0000 https://nsms10.com/josh-freed-two-teaspoons-of-wine-might-be-a-little-too-much-moderation/ Breadcrumb Links Opinion Columnists In the ever-changing world of studies, it’s time for alcohol to get mean. Author of the article: Josh freed • Montreal Gazette Special Easy, do you know how many teaspoons there are in this glass? Photo by Julie Jocsak /Postmedia files Content of the article DANGER: This beer may be harmful […]]]>

In the ever-changing world of studies, it’s time for alcohol to get mean.

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DANGER: This beer may be harmful to health. Recommended dose: two teaspoons.

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Could this be the label on liquor bottles in the years to come, with more and more talk of cigarette-style alcohol warnings? It’s such a happy thought, I might need a drink to deal with it.

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In the ever-changing world of studies, it’s time for alcohol to become a villain, a former health hero.

For many years, wine was practically a magical health elixir. According to early studies on the Mediterranean diet, two glasses of wine a day were considered excellent for your health, and three possibly better. Fortunately, I drank most of my drinks back then, when it was still good for us.

But as endless risk studies gradually envelop the planet, almost everything has been found to present some statistical danger, from eating rich foods to sunbathing, playing contact sports and alcohol consumption.

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Recently we saw two more studies on the dangers of alcohol. Study 1 was a not-at-all-fun Korean study that made headlines. He urged us to limit ourselves to six drinks a week, but warned that any increase in alcohol consumption somewhat increases our risk of certain cancers.

Frankly, this is a dismal study that its authors admit has procedural flaws. So let’s ignore it and move on to Study 2, which isn’t quite as grim.

Funded by the Gates Foundation in 204 countries and territories, it revealed that moderate alcohol consumption is only dangerous for those under 40, who suffer more from car accidents, violence-related incidents and probably headaches the next morning.

The study recommends that men under 40 limit themselves to two teaspoons of wine or 2 1/2 tablespoons of beer a day — honest!

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And that sound you hear in the background is several hundred million men in 204 countries laughing their heads off over a beer after work.

Even the lead author of the study says their suggestion may not be realistic.

Women under 40 experience fewer hazardous alcohol incidents and are therefore permitted to take twice daily soup spoons of wine, allowing them to drink men under the table.

This teaspoon diet could be difficult for restaurant sommeliers when they pour drinks to customers with a pipette and hiss: “Hold this teaspoon constantsir, it’s dripping down your lap.

However, like wine, things get better with age. At age 40, according to the study, you can start celebrating with a standard glass of wine a day without ill effects. At age 65, up to two drinks a day can reduce the risk of stroke and diabetes, and at age 70 you are allowed up to three drinks a day.

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It’s a rare age-related perk, along with cheap movie passes, free prescription drugs, and occasional restaurant discounts for early risers.

But we should take all of this with a teaspoon of salt, because health studies often swing like pendulums. For decades, coffee was bad for you; now it’s practically a miracle drink that some studies have shown may help ward off Alzheimer’s disease and increase longevity.

Likewise, for decades, eggs have been the healthiest breakfast ever. Then for two decades they were bad for you, and now they’re good for you again. For years electronic screens have been bad for kids, but during COVID we begged our kids to use them – and they practically saved our sanity.

Drinking alcohol is also confusing. For example, a “standard” size drink differs around the world, as time of day once did until Sandford Fleming introduced standard time.

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In England, Iceland and other countries, a standard drink is 8 grams of pure alcohol. In France and Italy it is 10 grams (a small glass of wine), in Denmark 12.

In Canada it is a hefty 13.45 grams, in Hungary 17, in Japan 19 and in Austria a whopping 20. So if possible, for health reasons, it is better to drink in Austria.

The truth is that life always involves risks and we have to find a reasonable balance between them, whether it’s skiing, horseback riding, climbing a ladder, driving a car or drinking.

There are also many things we know we are supposed to do: eat five to ten vegetables a day, walk 10,000 steps, and drink 100,000 glasses of water a day. We would like to behave well, but we also like to enjoy life.

All things in moderation, especially moderation. So if you are under 40, do not hesitate to exceed your two teaspoons of wine. But if you’re over 70, you don’t have to drink three.

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Full disclosure: When it comes to alcohol, I’m not the most objective source. I drank a cognac most nights of my life, although there were extenuating circumstances.

About fifteen years ago, during a conversation with my mother, she revealed to me that I was not a good sleeper as a baby, until the family doctor offered a solution wonderful.

He recommended giving me up to two teaspoons of brandy every night, which I drank until I was two years old, and apparently slept “like a baby.” If only all our behaviors were so easily explained.

Obviously I’m not advising this to mothers today, but considering I was under 40 at the time, those two teaspoons given by my mother meant she was ahead of her time. .

joshfreed49@gmail.com

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Distilling Billy: Tattersall launches a spirit collaboration with Billy Sushi https://nsms10.com/distilling-billy-tattersall-launches-a-spirit-collaboration-with-billy-sushi/ Tue, 06 Sep 2022 20:01:31 +0000 https://nsms10.com/distilling-billy-tattersall-launches-a-spirit-collaboration-with-billy-sushi/ It all started over a drink. As things do. Bentley Gillman, Tattersall’s chief distiller, is a bit of a mad scientist who deals with side projects that may or may not see the fast lane of the distillery’s production line. One of the projects he was working on earlier this year was a shochu-style rice-based […]]]>

It all started over a drink. As things do.

Bentley Gillman, Tattersall’s chief distiller, is a bit of a mad scientist who deals with side projects that may or may not see the fast lane of the distillery’s production line. One of the projects he was working on earlier this year was a shochu-style rice-based spirit.

Shochu is a distilled spirit whose traditions date back 500 years in Japan. Typically, it is made from rice, but barley, sweet potatoes, and buckwheat are also used. It typically packs a punch, with an ABV of around 25-30%, which is lower than whiskey but higher than sake (which is fermented with rice, while shochu is distilled). Korean soju is also distilled rice wine, but it generally contains less alcohol and may contain flavorings and additives beyond the distillate. In Japan, shochu is more popular than sake, accounting for 15% of alcohol consumption in 2020.

Gillman wanted to test the flavor of his project on the road, so why not speak to an expert. Along with Taylor Stein, Tattersall’s sales manager, Gillman brought it to a dinner party they had planned at Billy Sushi. Cautiously, they offered Billy Tserenbat, the iconic and intrepid Mongolian sushi maker, a sip.

“I thought it was impossible man,” Billy said when told that someone was making shochu in the area, “This can’t be good, this guy is crazy to think he can do it But then I tasted and realized he was the right kind of crazy. It was so good! If you saw Billy in full Mongolian dress sitting next to the A-rod at the Timberwolves game, you know that smart fools recognize smart fools.

So the two began their journey to create the perfect recipe for what is now known as The spirit of Sushiman. It’s not shochu in the strict sense, it’s more inspired by the process.

Gillman and his team spend a lot of time making rice balls. The labor intensive process is one of the reasons they are limiting production to around 500 bottles for this first round.

Gillman breaks it down as follows: “The rice is cooked and cooled and inoculated with rice yeast balls known as jui qu, jing bao or simply qu. These balls contain a combination of yeast, mold and bacteria that work together to liquefy, saccharify (convert starch into simple sugar), and ferment rice grains.The liquid produced from this process is similar to rustic sake, although sake production involves a few additional steps. It most closely resembles rural Chinese rice wine called mijiu, and Korean rice wine called cheongju or makgeolli, or rural Himalayan rice beer called chhaang at this point.It is tart, with a subtle, alcoholic sweetness. From there, this beer/rice wine is double distilled in the spirit of Sushiman. This finished product is called soju in Korea, shochu in Japan, shaojiu in China and raksi in Nepal.”

Billy and Gillman worked the recipe together, refining the technique to create a spirit that is clean, clear and easily sipped on its own or in cocktails. Stein recalled when he first started tasting shochu, how hot some spirits were when they were only around 25% alcohol. “The alcohol burn always surprised me and it made some of them harder to work with in cocktails, so I started drinking more rice-based liquors and thinking about how we could tame that burn.”

“I think it’s perfect right now,” Billy says. “We played with wild rice and buckwheat and it was so awesome.”

Gillman adds, “It’s challenging enough to get people to try new things like this, but once they get a taste of it, I think they’ll really love it.” And from there, maybe we can play around with funkier flavors.

After sipping the spirit, I can say it’s definitely not too hot on the tongue and has a much richer mouthfeel than anything misnamed Japanese vodka, like shochu l ‘is often. I would say almost creamy. It tastes very clean and carries just a bit of a floral, sweet sweetness that makes it an ideal sip to accompany sushi. It also layers well with a few cocktail creations I’ve tried, especially the Bee’s Knees with Honey Ginger Syrup. But I loved it in the highball with acidified lemongrass syrup, if I felt like the right light undertone in this bubbly refreshment.

You can of course find Spirit of Sushiman at Billy Sushi in North Loop. If he doesn’t have it in a hip holster by the end of the week, color me shocked. It might be your best bet to taste before buying a bottle, which will cost around $45 at local retailers. Surdyk’s will host Billy on September 10 from 2-4 p.m., where he will pour samples and sign bottles.

But if you really want the full Sushiman experience, make plans (read: find a driver) for the official bottle launch on September 22 at the Tattersall River Falls facility. At the 6-9pm launch, Billy will demonstrate how to break down a whole bluefin tuna, you’ll have small snack plates, a sample of the neat spirit and in three different cocktails specially created by the founder of Tattersall, Dan Oskey: There will be the Bee’s Knees riff, a Sushiman Daiquiri, and the highball version called Billy Collins. Tickets are $75/person and seating is limited, so if you’re hoping for a little crazy-smart to rub off on you, don’t delay.

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